Capetonian,
Remote host said: 550 #5.1.0 Address rejected
Strongly implies that there are "no problems with the domain", ie. the routing appears to be getting there, but it is "their" server that's saying "go away".
Based on that message, any issues are likely to be localised to the ISP's servers (i.e. maybe there were some fat-fingered configuration changes).
Ask the people you are sending to to give the ISP support desk a call.