mikepops
I'm not trying to rub salt into the wound but 'headyheights' has given a sound analysis of the situation and good advice. You were fortunate (in my experience) to get a reply to your initial application and if the owner of the trainset says he has got enough pilots then he has got enough pilots. Take it on the chin and mail him again in a few months time to see if his glut of pilots have all disappeared elsewhere.
Confrontation at your level of experience is a definate no,no. That can come when you are a CEO and then you can confront who the hell you like......but even then, be selctive!