will it happen in aircraft also in case of a real engine failure?
According FCOM - yes. (Though I've not had a real engine failure on A320).
This is actually an ECAM glitch.
ENG 1(2) START VALVE NOT OPEN
If opposite engine running : - X BLEED .....ON
FLT Phase INHIB 3,4,5, 7, 8.
If taking off with APU Bleed on (for Packs), then you shouldn't see this fault reported.
If taking off without APU Bleed, then ECAM is reminding you that the engine couldn't be cranked over by the starter motor unless you opened the X Bleed.
I don't know why the message isn't removed when the ENG Master Switch is put to off.