Never Fretter, an verage of 30% of EMS flights is flown at night in the USA.
The pilots are required to show up rested for night duty.
Normal EMS rotations are 12 hours day shifts and 12 hours night shifts (ie: 07:00-19:00 and 19:00-07:00).
Under part 135.267 unscheduled one pilot operations, minimum rest undisturbed has to be 10 hours in the last 24 hours, with a maximum duty of 14 consecutive hours.
That rest includes driving back and forth from the pilot's domicile.
The pilots are responsible to get their rest once they get home or to their "luxury trailer"