They were instructed by an ATCO 3 times to vacate, they did not comply.
No strictly true. The first two times they were at the holding point for 28 (at 00.20 and 00.28). Surely you cannot refuse to vacate a runway that you haven't entered.
For the ATCOs;
Is it acceptable to issue a 16 second admonishment while the aircraft is lining up for take-off? How would you feel if a pilot did the same when you were about to make a critical transmission?