If you install an application under Windows, it will place the actual files wherever you tell it to, and write entries in the registry accordingly.
As long as you do not alter the registry for that instance of the OS, then you can move the actual files anywhere you like and finally copy / move them back to their original location and the application will start correctly.
Obviously you can't expect the application to work until you have restored them to their original location!
By original location I mean a drive with the same drive letter as per the installation. So if you installed them to the G: drive, then a brand new disk as the G: drive will work fine when the files are restored to it. The position on the disk is irrelevant, as is the presence of any other files, including a complete operating system.
Probably the only gotcha is ensuring that file & directory permissions are correct - providing that defaults were left in place then this should be a non-issue.
SD