Can Mutt, Doug N, Clrdrvr, and the various other chaps who are thinking the answer is obvious give specific answers to these questions.
On an all engine operating take off do you retract flaps at 400 feet (presumably with V2+x)?
If the answer is yes, what data are you using for the climb from that point?
Please can you post a reference for any manual that you've used to show where the data is so I can have a look.
ta