The major airlines and some of their affiliates tend to be rigid about minimum educational standards. You won't be able to complete the initial on-line application if you can't tick all the boxes.
Smaller operators like Skippers play it more loose. Their requirements vary with supply and demand (tighter when there is an over supply, looser when there is a shortage). Often it will be their clients who drive the minimum flying experience level and that will over ride any educational standard.