No. Unless you live in the USA.
I not only don't have a college degree, I dropped out from the 3rd year of a 4 year university degree (Bachelor of Arts - Philosophy major!!) after I failed most of my courses miserably, largely due to drinking and playing the guitar too much when I should have been studying. I now have a 3 year gap in my "education" between high school and flying school that I have to explain
I subsequently applied for, and got, interviews with several big european and asian major airlines, including probably the most renowned hardest interview on earth - Cathay Pacific - and it wasn't even asked about. (Although I did make a complete monkey of myself in the sim assessment and wasn't offered the job). C'est la vie, I did a couple years with Easyjet instead and then moved on to my current position happily flying airbus widebodies with a middle eastern mega-carrier, who also didn't ask or care about my college degree or lack of.