Put simply, in light GA, highly unlikely.
You should be monitoring progress and prepare to abort take-offs is things are not going as planned but I would doubt many do a W&B or take-off performance calculation. Look at the case of the Diamond Twin Star at Lands End, as an example.
But unless there is a big bang pilots do have a habit of hanging on hoping things get better.
Mind you even glider pilots get it wrong. I've seen some waiting for the winch to accelerate when they should have pulled off earlier.
We are all very good at it when students, but bad habits do creep in over time.