Yes but consider your options BEFORE you enter the runway. "Ready for departure" really is what it means. 2 minutes WT separation is measured from airborne-time to airborne-time, not from airborne-time to clearance received/start-rolling-time. I never refused additional separation when requested by the pilot. But if you ask for it when already lined-up on the RWY and there is landing TFC 6 miles out you'll have to vacate the RWY and join the queue at the end.