1. Boeing tests takeoff performance without spoilers or aileron deflected. If you're not on the drag profile they certified, especially on a balanced field, you're a test pilot and on your own.
2. They recommend aileron as necessary to keep the wings level.
3. A plane at rest needs no cross controls. At rotation it will need the same amount of cross controlling that it would have if you were landing.
4. Understand #3, observe the wing 'lifting', and gradually apply #2, not to exceed #3.