You can get this problem on Notebooks, especially the ones with a swappable CDROM/Floppy drive as the PC will try and find the Floppy drive occasionally, as it's not plugged in, it takes about 10 secs to discover that.
There are a few fixes for this (I can't remember all of them!)
One of them is to clear the documents list (recent files).
Another is to clear the Start Menu's Run Command list.
Remove all PIF files that point to the floppy drive.
Remove LocalLoadHigh=1 from your MSDOS.SYS file.
There is a help sheet on the web about this, but I've lost the address, Oooops! Ah ha! Here it is:
www.annoyances.org/win95/win95ann6.html#07
That's your lot!
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400 Hertz but DC is easy