Favourite line when watching an aviation film (not in the film itself). Happened about 20 minutes into the execrable remake of "The Flight of the Phoenix". (The original is one of my all-time favourites).

Mrs TTN - "Are you seriously going to sit and watch any more of this rubbish?"

I didn't.

passenger 57
 

(rane bursts into the cockpit)
who's in charge?
(capt whitehurst)
i am.
(rane shoots whitehurst and says to the f.o. who's in charge?)
f.o. says you are.
(rane) excellent.

Dastardly and Muttley in their flying machines
 

Dick Dastardly: Muttley! Doooooo sooomethiiiiiinnnggg!

[theme song]
Dick Dastardly: Stop the pigeon, / Stop the pigeon, / Stop the pigeon, / Stop the pigeon, / Stop the pigeon, / Stop the pigeon. / Owwwww, nab him, jab him, tab him, grab him, stop that pigeon now!

Dick Dastardly: [after every attempt fails] Drat! And Double Drat!

Dick Dastardly: [Every time Klunk speaks to Dastardly] What'd he say? What'd he say?

and of course Muttley's snigger :ok:

Ice Station Zebra
 

Rock Hudson's character says,

"We're on a first name basis here. My name is Captain."

"Malta Story"

Alec Guiness in his PR Spitfire about to be bounced by numerous ME109s "this could get tricky"

"Top Gun"

Back seater to Tom Cruise "Come on Maverick, give me some of that pilot ****"

Not from a film, but from the 'Junkyard' pinball machine which I 'babysit' for a friend, who collects them and has run out of space:

"You're going down, FLYBOY!!!"

"I've personally flown over a hundred-ninety-four missions and I was shot down in every one of 'em. Come to think of it, I've never landed a plane in my life."
- Admiral Benson (Hot Shots!)

Has to be the Helicopter pilot in Apocalypse Now !!
 

Holy ****, she's a gook assassin, lemme nail that bitch, ****, I’m hit, I’m hit, I’m OK, Mayday, mayday, I’m going in

Susannah York
 

Has to be Susannah York in B of B:

"Don't you shout at me Mr Warwick!

Dam Busters:
'Can I have your egg if you don't come back'?