Not sure about the prop rotation direction or the power setting used during takeoff, but the engine position will have been chosen carefully for a number of reasons:
1. The engine can generate significantly more thrust then a standard herc engine, thus to minimise asymmetric effects it is best to have it near to the fuselage (No.2 or No.3 engine positions). The same applies for structural considerations such as wing bending moments. Look a photos of the test aircraft and you will see an additional support strut between the engine and the fuselage. Lastly, there is the issue of moments of inertia (roll and yaw) and lateral asymmetry (which can adversely affect roll and yaw control). Given the additional mass and thrust of the test engine compared to a standard herc engine it is far better to have the test engine nearer to the fuselage in order to minimise the change in inertias and thrust moments.
2. Being a test engine there is a risk it could fail unexpectedly, so you really do not want it on the No.1 or No.4 positions due to the large yawing moment that a sudden failure would cause.
3. In addition to the above there will have been some good engineering reasons for putting the engine on the No.2 position as opposed to the No.3 position, for example to minimise the change in yawing moment caused by the engine compared to the original engine. The position chosen will be the one that minimises the nasty aerodaynamic effects in the event of an engine failure. Incidentally, anyone care to state which engine is the critical engine on a Herc K?
Hope that goes some way to answering your questions!
WF